Anticorps Polyclonal de lapin anti-FN3K

FN3K Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14293-1-AP

Synonymes

FN3K, fructosamine 3 kinase

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Galerie de données de validation

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  WB analysis of HeLa using 14293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14293-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14293-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14293-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14293-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14293-1-AP

  IP result of anti-FN3K (IP:14293-1-AP, 3ug; Detection:14293-1-AP 1:500) with HeLa cells lysate 1840ug.

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  IHC staining of human kidney using 14293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14293-1-AP

  Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque humain, tissu cérébral humain, tissu de muscle squelettique de souris, tissu rénal humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14293-1-AP cible FN3K dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FN3K Protéine recombinante Ag5617
• Nom complet: fructosamine 3 kinase
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC042680
• Symbole du gène: FN3K
• Identification du gène (NCBI): 64122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	iScience; Amiloride reduces fructosamine-3-kinase expression to restore sunitinib sensitivity in renal cell carcinoma; Authors - Yuanyuan Bai