• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-FMR1

FMR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

N° de cat : 13755-1-AP

Synonymes

POF, FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1



Applications testées

Résultats positifs en WBcellules K-562, cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral de souris, tissu de thymus de souris
Résultats positifs en IPcellules HeLa
Résultats positifs en IHCtissu de côlon humain, tissu de cancer du côlon humain, tissu de gliome humain, tissu hépatique humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2, cellules HeLa
Résultats positifs en FC (Intra)cellules Jurkat,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13755-1-AP cible FMR1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène FMR1 Protéine recombinante Ag4697
Nom complet fragile X mental retardation 1
Masse moléculaire calculée 632 aa, 71 kDa
Poids moléculaire observé 59-72 kDa
Numéro d’acquisition GenBankBC038998
Symbole du gène FMR1
Identification du gène (NCBI) 2332
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocole

Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-APDownload protocol
IHC protocol for FMR1 antibody 13755-1-APDownload protocol
IF protocol for FMR1 antibody 13755-1-APDownload protocol
IP protocol for FMR1 antibody 13755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanWB

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang
humanWB

Cancer Cell Int

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

Authors - Yu Men
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
humanWB,IHC

Cell Death Dis

FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner

Authors - Yuhan Hu
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parisa (Verified Customer) (09-26-2023)

We used for WB, nor high specificity as we could see different bands (they could be different isoforms of the protein). But it was sensitive and we could see the band pulled down in IP for our protein of interest.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
FH

Marta (Verified Customer) (02-24-2020)

not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Brain mouse and rat
FMR1 Antibody Western Blot, validation (1/1000 dilution) in Brain mouse and rat (Cat no:13755-1-AP)