Anticorps Polyclonal de lapin anti-FMO5-specific

• Phare
• Validé par KD/KO

FMO5-specific Polyclonal Antibody for WB, IHC, ELISA, IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA, IF/ICC, FC (Intra)

Conjugaison

Non conjugué

Publications

2

N° de cat : 16864-1-AP

Synonymes

FMO5, Flavin-containing monooxygenase 5, EC:1.6.3.1, EC:1.14.13.8, EC:1.14.13.-

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Galerie de données de validation

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  WB analysis using 16864-1-AP

  PC-3 cells and LNCap cells were subjected to SDS PAGE followed by western blot with 16864-1-AP (FMO5-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16864-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16864-1-AP (FMO5-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 16864-1-AP

  WB result of 16864-1-AP from Dr.Soory.

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  IHC staining of human liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16864-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 16864-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 16864-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug FMO5-specific Polyclonal antibody (16864-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules DU 145, cellules LNCaP, cellules MCF-7, cellules RAW 264.7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du sein humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16864-1-AP cible FMO5-specific dans les applications de WB, IHC, ELISA, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: flavin containing monooxygenase 5
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_001461
• Symbole du gène: FMO5
• Identification du gène (NCBI): 2330
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB,IHC	Drug Metab Dispos; Identification of flavin-containing monooxygenase 5 (FMO5) as a regulator of glucose homeostasis and a potential sensor of gut bacteria.; Authors - Flora Scott ; KO Validated
mouse	IF	J Transl Med; Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism; Authors - Yi-Xi Li