Anticorps Polyclonal de lapin anti-FLNB

FLNB Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20685-1-AP

Synonymes

ABP 278, ABP 280 homolog, Actin binding like protein, AOI, Beta filamin, FH1, Filamin 3, Filamin B, Filamin homolog 1, FLN B, FLN1L, FLN3, FLNB, LRS1, SCT, TABP, TAP, Thyroid autoantigen, Truncated ABP

---

Galerie de données de validation

• 

  WB analysis of HeLa using 20685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 20685-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 20685-1-AP

  Immunofluorescent analysis of HepG2 cells, using FLNB antibody 20685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

20685-1-AP cible FLNB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: filamin B, beta (actin binding protein 278)
• Masse moléculaire calculée: 278 kDa
• Poids moléculaire observé: 250-278 kDa
• Numéro d’acquisition GenBank: NM_001457
• Symbole du gène: FLNB
• Identification du gène (NCBI): 2317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.

Publications

Species	Application	Title
human	WB	Cell Death Dis; NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.; Authors - Wenwen Chen
mouse	IHC	J Genet Genomics; Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts; Authors - Wenbin Huang
human,mouse	IHC,IF	Am J Cancer Res; FLNB overexpression promotes tumor progression and associates with immune suppression, evasion and stemness in pancreatic cancer; Authors - Guoyong Fan