Anticorps Polyclonal de lapin anti-FKBP8

• Phare
• Validé par KD/KO

FKBP8 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 11173-1-AP

Synonymes

38 kDa FK506 binding protein, 38 kDa FKBP, FK506 binding protein 8, FK506 binding protein 8, 38kDa, FKBP 38, FKBP 8, FKBP38, FKBP8, FKBPr38, hFKBP38, PPIase FKBP8, Rotamase

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Galerie de données de validation

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  WB analysis using 11173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11173-1-AP

  WB result of FKBP8 antibody (11173-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.

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  WB analysis of HeLa using 11173-1-AP

  WB result of FKBP8 antibody (11173-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.

• 

  WB analysis of Jurkat using 11173-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11173-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11173-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11173-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11173-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11173-1-AP

  IP result of anti-FKBP8 (IP:11173-1-AP, 4ug; Detection:11173-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of SH-SY5Y using 11173-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11173-1-AP (FKBP8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules SH-SY5Y, tissu cérébral de souris, tissu hépatique de souris, tissu pancréatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11173-1-AP cible FKBP8 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP8 Protéine recombinante Ag1649
• Nom complet: FK506 binding protein 8, 38kDa
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC009966
• Symbole du gène: FKBP8
• Identification du gène (NCBI): 23770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB,IP	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali
human		Front Bioeng Biotechnol; Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector; Authors - Suli Lv ; KO Validated
rat	IF	Int J Mol Sci; Analysis of Dysferlin Direct Interactions with Putative Repair Proteins Links Apoptotic Signaling to Ca2+ Elevation via PDCD6 and FKBP8; Authors - Dennis G Drescher
human	WB	J Biol Chem; Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma; Authors - Suli Lv ; KO Validated
human	WB	Mol Hum Reprod; Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance; Authors - Xiao Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells