Anticorps Monoclonal anti-FKBP52

FKBP52 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1B5F1

N° de cat : 66040-2-Ig

Synonymes

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

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Galerie de données de validation

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  WB analysis using 66040-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66040-2-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66040-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66040-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 66040-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 66040-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66040-2-Ig cible FKBP52 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FKBP52 Protéine recombinante Ag18704
• Nom complet: FK506 binding protein 4, 59kDa
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007924
• Symbole du gène: FKBP4
• Identification du gène (NCBI): 2288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.