Anticorps Polyclonal de lapin anti-FKBP5

• Phare
• Validé par KD/KO

FKBP5 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 14155-1-AP

Synonymes

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

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Galerie de données de validation

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  WB analysis of K-562 using 14155-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14155-1-AP

  IP result of anti-FKBP5 (IP:14155-1-AP, 3ug; Detection:14155-1-AP 1:1000) with K-562 cells lysate 1500ug.

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  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, tissu de thymus de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

14155-1-AP cible FKBP5 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP5 Protéine recombinante Ag5337
• Nom complet: FK506 binding protein 5
• Masse moléculaire calculée: 457 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC042605
• Symbole du gène: FKBP5
• Identification du gène (NCBI): 2289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
mouse	IHC	Nutrients; Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus; Authors - Tova M Bergsten
mouse	IHC	Mol Cancer Ther; Cell intrinsic role of COX-2 in pancreatic cancer development.; Authors - Hill Reginald R
mouse	WB	RNA; The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.; Authors - Martinez Natalia J NJ
mouse	WB	Biochem Pharmacol; Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.; Authors - Jordan M Buck