Anticorps Polyclonal de lapin anti-FKBP14

FKBP14 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15884-1-AP

Synonymes

22 kDa FK506 binding protein, 22 kDa FKBP, FK506 binding protein 14, FKBP 14, FKBP 22, FKBP14, FKBP22, PPIase FKBP14, Rotamase

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Galerie de données de validation

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  WB analysis of HEK-293 using 15884-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15884-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15884-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

15884-1-AP cible FKBP14 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FKBP14 Protéine recombinante Ag8668
• Nom complet: FK506 binding protein 14, 22 kDa
• Masse moléculaire calculée: 211 aa, 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005206
• Symbole du gène: FKBP14
• Identification du gène (NCBI): 55033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FKBP14, also named as FKBP22, belongs to the family of FK506-binding peptidyl-prolyl cis-trans isomerases (PPIases). PPIases accelerate the folding of proteins during protein synthesis. FKBP14 is localized in the endoplasmic reticulum (ER) and that deficiency of FKBP14 leads to enlarged ER cisterns in dermal fibroblasts in vivo. FKBP14 is about 22kd.

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.; Authors - Baumann Matthias M
human	WB,IF	Sci Rep; The novel missense mutation Met48Lys in FKBP22 changes its structure and functions.; Authors - Yoshihiro Ishikawa
human	IF	Hum Mutat; Kyphoscoliotic Ehlers-Danlos syndrome caused by pathogenic variants in FKBP14: Further insights into the phenotypic spectrum and pathogenic mechanisms; Authors - Birute Burnyte
human	WB	Int J Mol Sci; Local Net Charge State of Collagen Triple Helix Is a Determinant of FKBP22 Binding to Collagen III; Authors - Yoshihiro Ishikawa