Anticorps Polyclonal de lapin anti-FJX1

FJX1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17417-1-AP

Synonymes

FJX1, Four jointed box protein 1, Four jointed protein homolog

---

Galerie de données de validation

• 

  WB analysis of rat brain using 17417-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17417-1-AP (FJX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de rat,
• Résultats positifs en IHC: tissu cérébral de souris, tissu hépatique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

17417-1-AP cible FJX1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FJX1 Protéine recombinante Ag11385
• Nom complet: four jointed box 1 (Drosophila)
• Masse moléculaire calculée: 437 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC049171
• Symbole du gène: FJX1
• Identification du gène (NCBI): 24147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Four-jointed box protein 1 (FJX1), also named as FLJ22416, is a 437 amino acid protein, which belongs to the FJX1 family. FJX1 is a secreted protein and acts as an inhibitor of dendrite and branching. FJX1 is important for growth and differentiation of legs and wings, and for proper development of the eyes. FAT4, an essential gene that has a key role in vertebrate PCP (planar cell polarity), represses FJX1 expression which may lead to cystic diseases in humans.

Publications

Species	Application	Title
rat	WB	Mol Cell Endocrinol; Regulation of Sertoli cell function by planar cell polarity (PCP) protein Fjx1; Authors - Tiao Bu
rat	WB	Reprod Biol Endocrinol; Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin; Authors - Lingling Wang
human	WB	Endocrinology; The planar cell polarity (PCP) protein Fat 1 in Sertoli cell function; Authors - Tiao Bu