Anticorps Monoclonal anti-FITC

FITC Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

FITC

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3C10A1

N° de cat : 68132-1-Ig

Synonymes

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Galerie de données de validation

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  WB analysis using 68132-1-Ig

  WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein.

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  WB analysis of BSA using 68132-1-Ig

  WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA.

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  ELISA experiment of FITC conjugated antibody using 68132-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis).

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Applications testées

• Résultats positifs en WB: FITC-TDP43, FITC-Ag1231, BSA
• Résultats positifs en ELISA: FITC conjugated antibody

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Test immuno-enzymatique (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68132-1-Ig cible FITC dans les applications de WB, ELISA et montre une réactivité avec des échantillons FITC

• Réactivité: FITC
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Composé
• Nom complet: FITC
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.