Anticorps Monoclonal anti-FITC

FITC Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

FITC et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3C10A1

N° de cat : 68132-1-Ig

Synonymes

Fluorescein isothiocyanate

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Galerie de données de validation

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  WB analysis using 68132-1-Ig

  WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein.

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  WB analysis of BSA using 68132-1-Ig

  WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA.

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  ELISA experiment of FITC conjugated antibody using 68132-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis).

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Applications testées

• Résultats positifs en WB: FITC-TDP43, FITC-Ag1231, BSA
• Résultats positifs en ELISA: FITC conjugated antibody

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Test immuno-enzymatique (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

68132-1-Ig cible FITC dans les applications de WB, ELISA et montre une réactivité avec des échantillons FITC

• Réactivité: FITC
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: FITC
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Publications

Species	Application	Title
mouse	WB	Environ Health Perspect; Organophosphorus Flame Retardants and Metabolic Disruption: An in Silico, in Vitro, and in Vivo Study Focusing on Adiponectin Receptors; Authors - Ying Liu

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