Anticorps Polyclonal de lapin anti-FGFR1OP

• Phare
• Validé par KD/KO

FGFR1OP Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 11343-1-AP

Synonymes

FGFR1 oncogene partner, FGFR1OP, FOP

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Galerie de données de validation

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  WB analysis using 11343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11343-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 11343-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11343-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11343-1-AP (FGFR1OP antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11343-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 11343-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HL-60, cellules MCF-7, cellules U-937, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Informations sur le produit

11343-1-AP cible FGFR1OP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGFR1OP Protéine recombinante Ag1891
• Nom complet: FGFR1 oncogene partner
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC011902
• Symbole du gène: FGFR1OP
• Identification du gène (NCBI): 11116
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The fibroblast growth factor receptor 1 oncogene partner (FGFR1OP), FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.

Publications

Species	Application	Title
zebrafish	IF	Cell; Vertebrate cells differentially interpret ciliary and extraciliary cAMP.; Authors - Melissa E Truong
human	WB,IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu ; KD Validated
mouse	IF	Dev Cell; Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.; Authors - Chang Xie
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	IF	J Cell Biol; A ciliopathy complex builds distal appendages to initiate ciliogenesis.; Authors - Dhivya Kumar
human,mouse	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall