Anticorps Polyclonal de lapin anti-FGF-2

FGF-2 Polyclonal Antibody for WB, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

5

N° de cat : 11234-1-AP

Synonymes

Basic fibroblast growth factor, BFGF, FGF2, FGF-2, FGFB, HBGF 2

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Galerie de données de validation

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  WB analysis using 11234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11234-1-AP (FGF-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 11234-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of K-562 using 11234-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HepG2, cellules SKOV-3
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu cérébral de rat, tissu de cancer de la thyroïde humain, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules K-562, cellules HepG2
• Résultats positifs en cytométrie: cellules K-562, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

11234-1-AP cible FGF-2 dans les applications de WB, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fibroblast growth factor 2 (basic)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_002006
• Symbole du gène: FGF2
• Identification du gène (NCBI): 2247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

Publications

Species	Application	Title
mouse	IHC	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu
	WB	Biomaterials; Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury.; Authors - Dongwei Guan
human	WB	BMC Cancer; Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis; Authors - Yufei Wang
human	WB,IHC	Wound Repair Regen; Negative pressure wound therapy is associated with upregulation of bFGF and ERK1/2 in human diabetic foot wounds.; Authors - Shao-Ling Yang
human	WB, IHC	Exp Ther Med; Expression of signal transducer and activator of transcription 3 and its phosphorylated form is significantly upregulated in patients with papillary thyroid cancer.; Authors - L I Yan