Anticorps Polyclonal de lapin anti-FDPS

• Phare
• Validé par KD/KO

FDPS Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 16129-1-AP

Synonymes

FDPS, FPP synthase, FPPS, FPS, KIAA1293

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Galerie de données de validation

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  WB analysis using 16129-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16129-1-AP (FDPS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16129-1-AP

  WB result of FDPS antibody (16129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDPS transfected A431 cells.

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  WB analysis of rat liver using 16129-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16129-1-AP (FDPS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 16129-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16129-1-AP (FDPS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16129-1-AP

  IP result of anti-FDPS (IP:16129-1-AP, 4ug; Detection:16129-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, tissu de cervelet humain, tissu hépatique de rat
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 3 publications below

Informations sur le produit

16129-1-AP cible FDPS dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FDPS Protéine recombinante Ag9115
• Nom complet: farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
• Masse moléculaire calculée: 419 aa, 48 kDa
• Poids moléculaire observé: 39-41 kDa
• Numéro d’acquisition GenBank: BC010004
• Symbole du gène: FDPS
• Identification du gène (NCBI): 2224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression.

Publications

Species	Application	Title
human	WB	Cell Metab; Regulation of VKORC1L1 is critical for p53-mediated tumor suppression through vitamin K metabolism; Authors - Xin Yang ; KD Validated
mouse	WB, IHC	Cardiovasc Res; Cardiac-specific overexpression of farnesyl pyrophosphate synthase induces cardiac hypertrophy and dysfunction in mice.; Authors - Jian Yang
human	WB,IHC	EBioMedicine; Disruption of FDPS/Rac1 axis radiosensitizes pancreatic ductal adenocarcinoma by attenuating DNA damage response and immunosuppressive signalling.; Authors - Parthasarathy Seshacharyulu
	WB	J Biol Chem; Does Viperin Function as a Radical S-adenosyl-L-methionine-dependent Enzyme in Regulating Farnesylpyrophosphate Synthase Expression and Activity?; Authors - Caitlyn Makins
mouse	WB	Front Pharmacol; Transcriptomic Analysis Reveals the Protective Effects of Empagliflozin on Lipid Metabolism in Nonalcoholic Fatty Liver Disease.; Authors - Yuting Ma
mouse	WB	Int J Biochem Cell Biol; Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo.; Authors - Yang Jian J