Anticorps Polyclonal de lapin anti-FBXW11

• Phare
• Validé par KD/KO

FBXW11 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 13149-1-AP

Synonymes

BTRC2, BTRCP2, Fbw11, FBW1B, FBXW11, FBXW1B, Homologous to Slimb protein, Hos, KIAA0696

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Galerie de données de validation

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  WB analysis using 13149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 13149-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 13149-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 13149-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 13149-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 13149-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HCT 116 using 13149-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A431 using 13149-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 13149-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules SGC-7901, tissu d'estomac humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HCT 116, cellules A431, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

13149-1-AP cible FBXW11 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXW11 Protéine recombinante Ag3761
• Nom complet: F-box and WD repeat domain containing 11
• Masse moléculaire calculée: 542 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC026213
• Symbole du gène: FBXW11
• Identification du gène (NCBI): 23291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.; Authors - Jie Xu
human	WB	Cell Death Differ; NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.; Authors - Hao Li
human	WB,IP	EMBO J; The E3 ubiquitin ligase APC/CCdh1 degrades MCPH1 after MCPH1-βTrCP2-Cdc25A-mediated mitotic entry to ensure neurogenesis.; Authors - Xiaoqian Liu
	WB	Antioxidants (Basel); AMPK-Mediated Phosphorylation of Nrf2 at S374/S408/S433 Favors Its βTrCP2-Mediated Degradation in KEAP1-Deficient Cells; Authors - Eleni Petsouki
human	WB	Mol Cell Biol; DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.; Authors - Chen Hao H
human	WB	Front Oncol; uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.; Authors - Zhijian Zheng