Anticorps Polyclonal de lapin anti-Fibulin 5

• Phare
• Validé par KD/KO

Fibulin 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12188-1-AP

Synonymes

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

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Galerie de données de validation

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  WB analysis using 12188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12188-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12188-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12188-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 12188-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12188-1-AP

  IP result of anti-Fibulin 5 (IP:12188-1-AP, 3ug; Detection:12188-1-AP 1:300) with mouse heart tissue lysate 8000ug.

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  IHC staining of mouse lung using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.

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  IHC staining of mouse lung using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.

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  IHC staining of human pancreas using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cérébral de rat, tissu de muscle squelettique de souris, tissu placentaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu cardiaque humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12188-1-AP cible Fibulin 5 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fibulin 5 Protéine recombinante Ag2859
• Nom complet: fibulin 5
• Masse moléculaire calculée: 448 aa, 50 kDa
• Poids moléculaire observé: 55 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC022280
• Symbole du gène: Fibulin 5
• Identification du gène (NCBI): 10516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

Publications

Species	Application	Title
mouse	WB	Arterioscler Thromb Vasc Biol; Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.; Authors - Chen Ju-Yi JY
human	WB	Biochem J; Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.; Authors - Kapustin Alexander A
mouse	WB, IHC	Cell Tissue Res; Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.; Authors - Wenli Han
mouse	WB	Am J Physiol Cell Physiol; Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.; Authors - Varadarajan Sudhahar
human	WB	Clin Exp Pharmacol Physiol; Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.; Authors - Wei Zhang ; KD Validated
human	IF	JID Innov; Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.; Authors - Fumihiko Katagiri