Anticorps Polyclonal de lapin anti-FBLIM1

FBLIM1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13349-1-AP

Synonymes

CAL, FBLIM1, FBLP 1, FBLP1, filamin binding LIM protein 1, MIG2 interacting protein, Migfilin, RP11 169K16.5

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Galerie de données de validation

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  WB analysis of human heart using 13349-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13349-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13349-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 13349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

13349-1-AP cible FBLIM1 dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBLIM1 Protéine recombinante Ag3907
• Nom complet: filamin binding LIM protein 1
• Masse moléculaire calculée: 373 aa, 41 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC019895
• Symbole du gène: FBLIM1
• Identification du gène (NCBI): 54751
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBLIM1, also known as CAL, Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. FBLIM1 is a protein found in cell-cell and cell-ECM connections where it co-localizes with FLNA/C and FLNB. FBLIM1 was found to bind directly to FLNA/C and to be an important regulator of cell shape and motility. FBLIM1 exerts its influence on cellular functions by interacting with various binding partners; FLN via its N- terminal domain, VASP and Src via its proline-rich region, and kindlin-2 and the cardiac transcription factor, CSX/NKX2-5 via its C-terminal LIM domains. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. FBLIM1 serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. It is associated with actin stress fiber at cell-ECM focal adhesion sites. FBLP-1A and FBLP-1B are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. FBLP-1 is localized at actin stress fibers. FBLIM1 is implicated in cell shape modulation (spreading) and motility. FBLIM1 participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. It may also regulate the assembly of filamin-containing signaling complexes that control actin assembly. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. This antibody is a rabbit polyclonal antibody raised against full length human FBLIM1 antigen.

Publications

Species	Application	Title
human	IF	J Cell Sci; Functional differences between kindlin-1 and kindlin-2 in keratinocytes.; Authors - Bandyopadhyay Aditi A