Anticorps Polyclonal de lapin anti-FASTKD2

• Phare
• Validé par KD/KO

FASTKD2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 17464-1-AP

Synonymes

FAST kinase domains 2, FASTKD2, KIAA0971

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Galerie de données de validation

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  WB analysis using 17464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17464-1-AP

  WB result of FASTKD2 antibody (17464-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASTKD2 transfected HepG2 cells.

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  IP experiment of HepG2 using 17464-1-AP

  IP result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:1000) with HepG2 cells lysate 2040 ug.

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  IF Staining of U2OS using 17464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 17464-1-AP (FASTKD2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

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  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti- FASTKD2 (17464-1-AP) with FASTKD2-YFP overexpression in COS7 cells.

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  IF Staining of U2OS cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with U2OS cells.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IF/ICC: cellules COS7, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• RIP: See 1 publications below

Informations sur le produit

17464-1-AP cible FASTKD2 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FASTKD2 Protéine recombinante Ag11314
• Nom complet: FAST kinase domains 2
• Masse moléculaire calculée: 710 aa, 81 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC001544
• Symbole du gène: FASTKD2
• Identification du gène (NCBI): 22868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Publications

Species	Application	Title
human	IF,WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human	WB	Cell Metab; A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.; Authors - Jason D Arroyo
human	IF	Nucleic Acids Res; FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.; Authors - Erik Boehm
human	WB	EMBO Rep; A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.; Authors - Hana Antonicka
human	WB, IF, RIP	Cell Rep; Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.; Authors - Hana Antonicka ; KD Validated
	IF	Cell Rep; A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.; Authors - Alexis A Jourdain

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height

• Applications: Western Blot
• Cell Tissue Type: PANC1 and MIAPACA2 cell lines

FASTKD2 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:17464-1-AP)