Anticorps Polyclonal de lapin anti-FARSA

• Phare
• Validé par KD/KO

FARSA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18121-1-AP

Synonymes

CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS

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Galerie de données de validation

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  WB analysis using 18121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18121-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18121-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 18121-1-AP

  IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug.

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  IHC staining of human liver cancer using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 18121-1-AP

  Immunofluorescent analysis of MCF-7 cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 18121-1-AP

  Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and FITC- labeled Donkey anti-Rabbit IgG (green).

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  IF Staining of HeLa using 18121-1-AP

  Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:4000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

18121-1-AP cible FARSA dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FARSA Protéine recombinante Ag12647
• Nom complet: phenylalanyl-tRNA synthetase, alpha subunit
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC006495
• Symbole du gène: FARSA
• Identification du gène (NCBI): 2193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Clin Genet; FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs!; Authors - Luise A Schuch
human,rat,mouse	IF,ELISA	Nat Commun; Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.; Authors - Qian Zhou ; KO Validated
human	WB,IP	Autoimmun Rev; Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase; Authors - Yoshinao Muro
human,mouse	WB,IF,RIP	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj