Anticorps Polyclonal de lapin anti-FAM96B

• Phare
• Validé par KD/KO

FAM96B Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 20108-1-AP

Synonymes

CGI 128, CIAB, CIAO2B, FAM96B, HSPC118, MIP18, Protein FAM96B

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Galerie de données de validation

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  WB analysis using 20108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20108-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 20108-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20108-1-AP

  IP result of anti-FAM96B (IP:20108-1-AP, 4ug; Detection:20108-1-AP 1:300) with K-562 cells lysate 2400 ug.

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  IHC staining of mouse kidney using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, cellules Ramos
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu rénal de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• CoIP: See 1 publications below

Informations sur le produit

20108-1-AP cible FAM96B dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM96B Protéine recombinante Ag13827
• Nom complet: family with sequence similarity 96, member B
• Masse moléculaire calculée: 163 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC001733
• Symbole du gène: FAM96B
• Identification du gène (NCBI): 51647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM96B, also known as MIP18 or CIAO2B (Cytosolic iron-sulfur assembly component 2B), is a component of the cytosolic iron-sulfur protein assembly (CIA) complex involved in iron homeostasis and part of the MMXD complex contributed to proper chromosome segregation (PMID: 36503010). FAM96B may act as a scaffold protein to recruit CKB and the other components of the MMXD complex to associate with the spindle and mitotic apparatus and contributes to chromosome segregation.

Publications

Species	Application	Title
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling ; KD Validated
human	WB	Nat Struct Mol Biol; Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.; Authors - Nima N Naseri
human	WB	Cell Metab; Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.; Authors - Stehling Oliver O ; KD Validated
human	WB	Mol Cell; An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis.; Authors - Adarsh K Mayank
human	WB	Cell Rep; The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.; Authors - Diana C Odermatt
human	WB	J Biol Chem; The Association of the DNA helicase XPD with Transcription Factor IIH is regulated by the Cytosolic Iron-sulfur Cluster Assembly Pathway.; Authors - Ajay A Vashisht