Anticorps Polyclonal de lapin anti-FAM84B

• Phare
• Validé par KD/KO

FAM84B Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 18421-1-AP

Synonymes

FAM84B, NSE2, Protein FAM84B, Protein NSE2

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 18421-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18421-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 18421-1-AP

  IP result of anti-FAM84B (IP:18421-1-AP, 4ug; Detection:18421-1-AP 1:300) with HUVEC cells lysate 3600ug.

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  IHC staining of human liver cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human oesophagus cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 18421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s, cellules Jurkat
• Résultats positifs en IP: cellules HUVEC
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu cutané humain, tissu de cancer de l'œsophage humain, tissu hépatique humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

18421-1-AP cible FAM84B dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM84B Protéine recombinante Ag13243
• Nom complet: family with sequence similarity 84, member B
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC052957
• Symbole du gène: FAM84B
• Identification du gène (NCBI): 157638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Nat Genet; Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism.; Authors - Zheng Hu
human	WB,IHC	Gigascience; Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.; Authors - Caixia Cheng ; KD Validated
human	IF	Proc Natl Acad Sci U S A; An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.; Authors - Yan Huang ; KD Validated
human	WB,IHC	Ther Adv Med Oncol; FAM84B promotes prostate tumorigenesis through a network alteration.; Authors - Yanzhi Jiang
human	IHC	Aging (Albany NY); FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway.; Authors - Xin Zhang
human,mouse	WB,IHC	Oncotarget; Upregulation of FAM84B during prostate cancer progression.; Authors - Nicholas Wong