Anticorps Polyclonal de lapin anti-FAM65B

• Phare
• Validé par KD/KO

FAM65B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17015-1-AP

Synonymes

C6orf32, DIFF40, DIFF48, FAM65B, KIAA0386, PL48, Protein FAM65B, RIPOR2

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 17015-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17015-1-AP (FAM65B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 17015-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17015-1-AP (FAM65B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

17015-1-AP cible FAM65B dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM65B Protéine recombinante Ag9991
• Nom complet: family with sequence similarity 65, member B
• Masse moléculaire calculée: 118 kDa
• Poids moléculaire observé: 115 kDa, 66 kDa
• Numéro d’acquisition GenBank: BC001232
• Symbole du gène: FAM65B
• Identification du gène (NCBI): 9750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.

Publications

Species	Application	Title
mouse, rat	WB,IF	Proc Natl Acad Sci U S A; FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.; Authors - Oscar Diaz-Horta
mouse	WB	J Cell Sci; Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.; Authors - Kun Gao ; KO Validated
human	WB	FASEB J; Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.; Authors - Anuradha Balasubramanian