Anticorps Polyclonal de lapin anti-Piezo1 (extracellular domain)

• Phare
• Validé par KD/KO

Piezo1 (extracellular domain) Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

135

Avis (7)

N° de cat : 15939-1-AP

Synonymes

FAM38A, KIAA0233, Mib, PIEZO1, Piezo1 (extracellular domain)

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Galerie de données de validation

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  WB analysis of HeLa using 15939-1-AP

  WB result of Piezo1 antibody (15939-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-Piezo1 transfected HeLa cells.

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  WB analysis using 15939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15939-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15939-1-AP (Piezo1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15939-1-AP (Piezo1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of THP-1 using 15939-1-AP

  Immunofluorescent analysis of THP-1 cells using Piezo1 (extracellular domain) antibody (15939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of THP-1 using 15939-1-AP

  Immunofluorescent analysis of THP-1 cells using Piezo1 (extracellular domain) antibody (15939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules hTERT-RPE1, cellules SGC-7901
• Résultats positifs en IHC: tissu cutané humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 32 publications below
• WB: See 91 publications below
• IHC: See 33 publications below
• IF: See 67 publications below
• FC: See 4 publications below
• CoIP: See 5 publications below

Informations sur le produit

15939-1-AP cible Piezo1 (extracellular domain) dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Piezo1 (extracellular domain) Protéine recombinante Ag7791
• Nom complet: family with sequence similarity 38, member A
• Masse moléculaire calculée: 286 kDa
• Poids moléculaire observé: 233-286 kDa
• Numéro d’acquisition GenBank: BC008073
• Symbole du gène: Piezo1/FAM38A
• Identification du gène (NCBI): 9780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Publications

Species	Application	Title
zebrafish	IF	Nature; Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.; Authors - Keat Ying Chan ; KO Validated
mouse,zebrafish	WB	Nature; Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.; Authors - Eisenhoffer George T GT ; KD Validated
human	WB	Nature; Piezo1 integration of vascular architecture with physiological force.; Authors - Jing Li ; KD Validated
canine, zebrafish	WB,IF	Nature; Mechanical stretch triggers rapid epithelial cell division through Piezo1.; Authors - S A Gudipaty ; KO Validated
mouse	IHC	Cell; RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.; Authors - Erika Sugisawa
human	WB	Blood; Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.; Authors - ShengPeng Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hyojin (Verified Customer) (01-19-2024)

I like the quality of antibody. Thank you for generating.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:200 for IF 1:1000 for WB
• Cell Tissue Type: mouse/human

FH

Sammy (Verified Customer) (12-04-2023)

Ab detects a band at the correct size but the signal is weak. This works only when detected via HRP/Chemi. NIR-conjugated antibodies do not detect this band.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

Piezo1 (extracellular domain) Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:15939-1-AP)

FH

Xiaonan (Verified Customer) (02-01-2023)

We did not see the band for Piezol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse kidney

Piezo1 (extracellular domain) Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:15939-1-AP)

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-23-2020)

Works with one band. Due to the large protein size, band does not always show sharp image.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela cells

FH

Canzhao (Verified Customer) (01-17-2020)

It worked in western blot and showed nice band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse heart tissue

FH

Zeinab (Verified Customer) (09-27-2019)

Worked great, Clean and Specific

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Human Cardiac Microvascular Endothelial cells

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