Anticorps Polyclonal de lapin anti-FAM134B

• Phare
• Validé par KD/KO

FAM134B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

36

Avis (1)

N° de cat : 21537-1-AP

Synonymes

FAM134B, JK-1, Protein FAM134B, Reticulophagy receptor 1, RETREG1

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Galerie de données de validation

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  WB analysis of HEK-293 using 21537-1-AP

  WB result of FAM134B antibody (21537-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM134B transfected HEK-293 cells.

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  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules C2C12, cellules HEK-293, cerveau de souris, cervelet de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 34 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

21537-1-AP cible FAM134B dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAM134B Protéine recombinante Ag16176
• Nom complet: family with sequence similarity 134, member B
• Masse moléculaire calculée: 497 aa, 55 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC030517
• Symbole du gène: FAM134B
• Identification du gène (NCBI): 54463
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Combinatorial selective ER-phagy remodels the ER during neurogenesis; Authors - Melissa J Hoyer
human	WB	Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham ; KD Validated
human	WB	Nat Commun; Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.; Authors - Mark L Schultz
mouse	WB,IF	Dev Cell; Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development; Authors - Patrick Lüningschrör
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely
human	WB	EMBO J; CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.; Authors - Thaddaeus Mutugi Nthiga

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (04-06-2020)

The antibody works well. There were a few non-specific bands in HEK293T lysates though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FAM134B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:21537-1-AP)