Anticorps Polyclonal de lapin anti-FADS3

FADS3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 15205-1-AP

Synonymes

CYB5RP, Cytochrome b5 related protein, FADS3, fatty acid desaturase 3, LLCDL3

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Galerie de données de validation

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  WB analysis using 15205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15205-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 15205-1-AP

  IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules L02, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15205-1-AP cible FADS3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FADS3 Protéine recombinante Ag7355
• Nom complet: fatty acid desaturase 3
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 41-51 kDa
• Numéro d’acquisition GenBank: BC004901
• Symbole du gène: FADS3
• Identification du gène (NCBI): 3995
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.