Anticorps Polyclonal de lapin anti-FADS1

• Phare
• Validé par KD/KO

FADS1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10627-1-AP

Synonymes

D5D, Delta(5) desaturase, Delta(5) fatty acid desaturase, FADS1, FADS6, FADSD5, fatty acid desaturase 1, LLCDL1, TU12

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Galerie de données de validation

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  WB analysis using 10627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10627-1-AP (FADS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10627-1-AP (FADS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10627-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 10627-1-AP (FADS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HeLa, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10627-1-AP cible FADS1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FADS1 Protéine recombinante Ag0978
• Nom complet: fatty acid desaturase 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007846
• Symbole du gène: FADS1
• Identification du gène (NCBI): 3992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FADS1, also known as Delta-5 desaturase or D5D, is a 444 amino acid protein that belongs to the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS1 is a key enzyme in polyunsaturated fatty acid (PUFA) metabolism that catalyze the conversion of linoleic acid (LA) into arachidonic acid (AA) and that of alpha-linolenic acid (ALA) into eicosapentaenoic acid (EPA).

Publications

Species	Application	Title
human	WB	Cell Metab; STING orchestrates the crosstalk between polyunsaturated fatty acid metabolism and inflammatory responses.; Authors - Isabelle K Vila
human	WB	Nat Commun; The lipoprotein-associated phospholipase A2 inhibitor Darapladib sensitises cancer cells to ferroptosis by remodelling lipid metabolism; Authors - Mihee Oh
human	WB	Proc Natl Acad Sci U S A; Polyunsaturated fatty acid biosynthesis pathway determines ferroptosis sensitivity in gastric cancer.; Authors - Ji-Yoon Lee
human	IHC	Stem Cells; Increased lipogenesis is critical for self-renewal and growth of breast cancer stem cells: Impact of omega-3 fatty acids.; Authors - Haiqing Luo
mouse	WB	J Lipid Res; In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGF alpha/c-myc transgenic mouse model.; Authors - Griffitts J J
human	IHC	J Cancer; Reduced Expression of FADS1 Predicts Worse Prognosis in Non-Small-Cell Lung Cancer.; Authors - Dong Wang