Anticorps Polyclonal de lapin anti-FADD

FADD Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (1)

N° de cat : 14906-1-AP

Synonymes

FADD, GIG3, MORT1, Protein FADD

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Galerie de données de validation

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  WB analysis using 14906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14906-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14906-1-AP

  mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 14906-1-AP

  IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug.

• 

  IHC staining of human lung cancer using 14906-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14906-1-AP (FADD antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 14906-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14906-1-AP (FADD antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, moelle osseuse de souris, tissu pancréatique de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 37 publications below
• IHC: See 4 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

14906-1-AP cible FADD dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FADD Protéine recombinante Ag6701
• Nom complet: Fas (TNFRSF6)-associated via death domain
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-30 kDa
• Numéro d’acquisition GenBank: BC000334
• Symbole du gène: FADD
• Identification du gène (NCBI): 8772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Publications

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	WB	PLoS Biol; A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.; Authors - Yingying Zhang
human	WB	Eur J Med Chem; PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism; Authors - Hongyan Lin
rat	WB,IHC	Cancers (Basel); Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1; Authors - Tiantian Qiu
human	WB,IP	Int Immunopharmacol; EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis; Authors - Huiling Zhang
human	WB,CoIP	Cancer Biol Med; A causal variant rs3769823 in 2q33.1 involved in apoptosis pathway leading to a decreased risk of non-small cell lung cancer; Authors - Xu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Isha (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:800 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: kidney

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