Anticorps Polyclonal de lapin anti-FABP6

• Phare
• Validé par KD/KO

FABP6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

6

N° de cat : 13781-1-AP

Synonymes

FABP6, Fatty acid binding protein 6, Gastrotropin, GT, I 15P, I BABP, I BALB, I BAP, ILBP, ILBP3, Ileal lipid binding protein, ILLBP, Intestinal 15 kDa protein

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Galerie de données de validation

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  WB analysis of mouse small intestine using 13781-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13781-1-AP (FABP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris,
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

13781-1-AP cible FABP6 dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FABP6 Protéine recombinante Ag4788
• Nom complet: fatty acid binding protein 6, ileal
• Masse moléculaire calculée: 177 aa, 20 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC022489
• Symbole du gène: FABP6
• Identification du gène (NCBI): 2172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fatty acid binding protein 6 (FABP6, also known as the ileal bile acid binding protein IBABP) is regarded as a bile acid binding protein found in the distal portion of the small intestine and may be important in maintaining bile acid homeostasis(PMID: 25754072). FABP6 is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer(PMID: 17909007). And also, it was showed a potential drug target for the treatment of diabetes(PMID: 27500412). There are 2 isoforms of this protein,one of which is about 14 kDa we detected.

Publications

Species	Application	Title
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu
human	WB,IHC	J Immunol Res; FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells; Authors - Wenping Lian ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); FABP6 serves as a new therapeutic target in esophageal tumor; Authors - Dengfeng Zhang
human	WB	Int Immunopharmacol; Melatonin alleviates necrotizing enterocolitis by reducing bile acid levels through the SIRT1/FXR signalling axis; Authors - Jiahao Lai