Anticorps Monoclonal anti-Factor XII

Factor XII Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2E2F11

N° de cat : 66089-1-Ig

Synonymes

Factor XII, Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor

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Galerie de données de validation

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  WB analysis of K-562 using 66089-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 66089-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66089-1-Ig(Factor XII antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse liver using 66089-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66089-1-Ig

  Immunofluorescent analysis of HepG2 cells, using F12 antibody 66089-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: cellules K562, cellules K-562
• Résultats positifs en IHC: tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu hépatique de souris, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66089-1-Ig cible Factor XII dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Factor XII Protéine recombinante Ag3401
• Nom complet: coagulation factor XII (Hageman factor)
• Masse moléculaire calculée: 615 aa, 68 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC012390
• Symbole du gène: F12
• Identification du gène (NCBI): 2161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.; Authors - Fanfan Li