Anticorps Polyclonal de lapin anti-Factor XII

• Phare
• Validé par KD/KO

Factor XII Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12551-1-AP

Synonymes

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

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Galerie de données de validation

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  WB analysis using 12551-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12551-1-AP (Factor XII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12551-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12551-1-AP (Factor XII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12551-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12551-1-AP (Factor XII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12551-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12551-1-AP (Factor XII antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12551-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12551-1-AP (Factor XII antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12551-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12551-1-AP (Factor XII antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

12551-1-AP cible Factor XII dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Factor XII Protéine recombinante Ag3401
• Nom complet: coagulation factor XII (Hageman factor)
• Masse moléculaire calculée: 615 aa, 68 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC012390
• Symbole du gène: Factor XII
• Identification du gène (NCBI): 2161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells.; Authors - Kerstin Göbel
human	IHC	Thyroid; Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.; Authors - Kai Guo
mouse	WB,IHC	Int J Mol Med; FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice.; Authors - Yan Meng ; KD Validated
human	IHC	Breast Cancer (Dove Med Press); Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer; Authors - Shen Ma