Anticorps Polyclonal de lapin anti-EXOSC7

EXOSC7 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 25292-1-AP

Synonymes

EAP1, EXOSC7, exosome component 7, hRrp42p, KIAA0116, p8, RRP42, Rrp42p

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Galerie de données de validation

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  WB analysis of MCF-7 using 25292-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25292-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25292-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25292-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25292-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25292-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25292-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 25292-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 25292-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 25292-1-AP (EXOSC7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules COLO 320, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu rénal humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

25292-1-AP cible EXOSC7 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC7 Protéine recombinante Ag18212
• Nom complet: exosome component 7
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC012831
• Symbole du gène: EXOSC7
• Identification du gène (NCBI): 23016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA

Publications

Species	Application	Title
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB,IF,IP	Cell Rep; ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells; Authors - M Carmen Lafita-Navarro

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

40µg of total protein were resolved on the gel. Incubation was done ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

EXOSC7 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:25292-1-AP)