Anticorps Polyclonal de lapin anti-EXOC1

• Phare
• Validé par KD/KO

EXOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 11690-1-AP

Synonymes

SEC3P, SEC3L1, SEC3, Exocyst complex component Sec3, Exocyst complex component 1

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Galerie de données de validation

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  WB analysis of rat brain using 11690-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11690-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11690-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11690-1-AP

  IP result of anti-EXOC1 (IP:11690-1-AP, 3ug; Detection:11690-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human gliomas using 11690-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11690-1-AP (EXOC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of human placenta using 11690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using EXOC1 antibody (11690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDA-MB-231 using 11690-1-AP

  Immunostaining of exocyst protein Sec3 (11690-1-AP, blue) in MDA-MB-231 cells. The image was credited by Dr. Hae Lin Jang from Harvard Medical School.

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  IF Staining of C2C12 using 11690-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using EXOC1 antibody (11690-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,
• Résultats positifs en IF/ICC: cellules MDA-MB-231, cellules C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

11690-1-AP cible EXOC1 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOC1 Protéine recombinante Ag2303
• Nom complet: exocyst complex component 1
• Masse moléculaire calculée: 894 aa, 102 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC020650
• Symbole du gène: EXOC1
• Identification du gène (NCBI): 55763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
human	WB,IF	Nat Nanotechnol; Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.; Authors - Tanmoy Saha ; KD Validated
mouse	WB	J Cell Biol; The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.; Authors - Juan José Sáez
mouse	IF	Elife; EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.; Authors - Yuki Osawa
human	IF	PLoS Pathog; Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.; Authors - Yuen-Yan Chang
human	WB,IP	Retrovirology; Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation.; Authors - Mukerji Joya J