Anticorps Polyclonal de lapin anti-ETFDH

• Phare
• Validé par KD/KO

ETFDH Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11109-1-AP

Synonymes

ETF dehydrogenase, ETF QO, ETF ubiquinone oxidoreductase, ETFDH, ETFQO, MADD

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Galerie de données de validation

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  WB analysis using 11109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11109-1-AP

  WB result of ETFDH antibody (11109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETFDH transfected HEK-293 cells.

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  WB analysis using 11109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11109-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11109-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11109-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11109-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11109-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11109-1-AP

  IP result of anti-ETFDH (IP:11109-1-AP, 3ug; Detection:11109-1-AP 1:500) with mouse brain tissue lysate 8000ug.

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  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu de muscle squelettique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11109-1-AP cible ETFDH dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETFDH Protéine recombinante Ag1568
• Nom complet: electron-transferring-flavoprotein dehydrogenase
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC011890
• Symbole du gène: ETFDH
• Identification du gène (NCBI): 2110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) is also named as ETF-QO and belongs to the ETF-QO/fixC family.It is a 64-kDa monomer integrated in the inner mitochondrial membrane, con- tains one molecule of FAD and a 4Fe4S cluster(PMID:12815589).Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C).This antibody is speicific to ETFDH.

Publications

Species	Application	Title
human,mouse	WB	Ann Neurol; Both ETFDH mutations and FAD homeostasis disturbance are essential for developing riboflavin-responsive multiple Acyl-CoA dehydrogenation deficiency.; Authors - Jingwen Xu
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
human	WB	Hum Mol Genet; Clinical, pathological and genetic features and follow-up of 110 patients with late-onset MADD: A single-center retrospective study.; Authors - Bing Wen
human	WB	J Neurol Neurosurg Psychiatry; Riboflavin-responsive lipid-storage myopathy caused by ETFDH gene mutations.; Authors - Wen Bing B
human	WB,IF	Front Oncol; Propofol Inhibits Proliferation and Augments the Anti-Tumor Effect of Doxorubicin and Paclitaxel Partly Through Promoting Ferroptosis in Triple-Negative Breast Cancer Cells.; Authors - Chen Sun
human	WB	J Mol Med (Berl); Molecular analysis of 51 unrelated pedigrees with late-onset multiple acyl-CoA dehydrogenation deficiency (MADD) in southern China confirmed the most common ETFDH mutation and high carrier frequency of c.250G>A.; Authors - Wang Zhi-Qiang ZQ