Anticorps Monoclonal anti-ESYT1

ESYT1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2C12E4

Avis (1)

N° de cat : 67688-1-Ig

Synonymes

E Syt1, ESYT1, E-Syt1, Extended synaptotagmin 1, Extended synaptotagmin-1, FAM62A, KIAA0747, MBC2

---

Galerie de données de validation

• 

  WB analysis using 67688-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67688-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MOLT-4, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

67688-1-Ig cible ESYT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ESYT1 Protéine recombinante Ag16840
• Nom complet: family with sequence similarity 62 (C2 domain containing), member A
• Masse moléculaire calculée: 1104 aa, 123 kDa
• Poids moléculaire observé: 123 kDa
• Numéro d’acquisition GenBank: BC004998
• Symbole du gène: FAM62A
• Identification du gène (NCBI): 23344
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair; Authors - Arsila P. K. Ashraf ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Shihab (Verified Customer) (09-17-2021)

This antibody was used in a western blot application to identify recombinant Esyt1 prescence. Using 1:500 at the first attempt, this antibody was able to successfully provide a band at the correct size. Furthermore, the band was clean which was an added bonus. I will be reducing the concentration of antibody used and also trying this antibody in other applications as well as primary cell lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 (will be reducing)
• Cell Tissue Type: HEK293 transfected with Esyt1