Anticorps Polyclonal de lapin anti-ERG

• Phare
• Validé par KD/KO

ERG Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14356-1-AP

Synonymes

Transforming protein ERG, Transcriptional regulator ERG, erg 3

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Galerie de données de validation

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  WB analysis of MCF-7 using 14356-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14356-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14356-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14356-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14356-1-AP

  IP result of anti-ERG (IP:14356-1-AP, 4ug; Detection:14356-1-AP 1:500) with MCF-7 cells lysate 800ug.

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  IHC staining of human prostate cancer using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14356-1-AP (ERG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 14356-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human ERG (14356-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral de souris, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14356-1-AP cible ERG dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERG Protéine recombinante Ag5708
• Nom complet: v-ets erythroblastosis virus E26 oncogene homolog (avian)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC040168
• Symbole du gène: ERG
• Identification du gène (NCBI): 2078
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

Publications

Species	Application	Title
human	WB	Cell Biosci; Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer; Authors - Qiong Wang
human	WB	J Cancer; miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.; Authors - Yongbao Wei ; KD Validated
human,mouse	WB,IF	Cell Death Discov; HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.; Authors - Feng Yao
human	IHC	Exp Ther Med; Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report; Authors - Xiaohua Wang
human	WB	Nat Commun; DNA framework-engineered chimeras platform enables selectively targeted protein degradation; Authors - Li Zhou

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011