Anticorps Polyclonal de lapin anti-ERCC3

• Phare
• Validé par KD/KO

ERCC3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10580-1-AP

Synonymes

BTF2, BTF2 p89, ERCC3, GTF2H, RAD25, TFIIH, TFIIH 89 kDa subunit, TFIIH p89, XPB, XPBC

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Galerie de données de validation

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  WB analysis of HEK-293 using 10580-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10580-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 10580-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10580-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10580-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10580-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10580-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10580-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10580-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10580-1-AP

  IP result of anti-ERCC3 (IP:10580-1-AP, 4ug; Detection:10580-1-AP 1:500) with A431 cells lysate 1200ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules COLO 320, cellules HeLa, cellules HT-1080, tissu de côlon de souris
• Résultats positifs en IP: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

10580-1-AP cible ERCC3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERCC3 Protéine recombinante Ag0876
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC008820
• Symbole du gène: ERCC3
• Identification du gène (NCBI): 2071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].

Publications

Species	Application	Title
human	WB	Theranostics; Triptolide Inhibits the AR Signaling Pathway to Suppress the Proliferation of Enzalutamide Resistant Prostate Cancer Cells.; Authors - Yangyang Han ; KD Validated
human	WB	Biochem Biophys Res Commun; MiR-192 inhibits nucleotide excision repair by targeting ERCC3 and ERCC4 in HepG2.2.15 cells.; Authors - Xie Qiong-Hui QH
human	WB	Science; Structural visualization of transcription initiation in action; Authors - Xizi Chen
		Mol Cell; Three-step mechanism of promoter escape by RNA polymerase II; Authors - Yumeng Zhan