Anticorps Polyclonal de lapin anti-ERCC2

ERCC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10818-1-AP

Synonymes

BTF2 p80, COFS2, CXPD, EM9, ERCC2, TFIIH 80 kDa subunit, TFIIH p80, TTD, XPD, XPDC

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Galerie de données de validation

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  WB analysis of HeLa using 10818-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10818-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10818-1-AP

  IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human cervical cancer using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules K-562
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

10818-1-AP cible ERCC2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERCC2 Protéine recombinante Ag1038
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 2
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC008346
• Symbole du gène: ERCC2
• Identification du gène (NCBI): 2068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

Publications

Species	Application	Title
human	IHC	Am J Cancer Res; Predictive value of ERCC1, ERCC2, ERCC4, and glutathione S-Transferase Pi expression for the efficacy and safety of FOLFIRINOX in patients with unresectable pancreatic cancer.; Authors - Shun Tezuka
human	IHC	Methods; Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research.; Authors - Zhao Jin-yao JY
human	IHC	Cancer Sci; Very low prevalence of XPD K751Q polymorphism and its association with XPD expression and outcomes of FOLFOX-4 treatment in Asian patients with colorectal carcinoma.; Authors - Lai Jiun-I JI
human	WB	Oncotarget; PMID: 27449101; Authors - Henan Zhao
human	IHC	Biomarkers; Aberrant methylation of nucleotide excision repair genes is associated with chronic arsenic poisoning; Authors - Aihua Zhang
human	IHC	J Transl Med; Proteomic analysis predicts anti-angiogenic resistance in recurred glioblastoma; Authors - Hanwool Jeon

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zizhao (Verified Customer) (08-15-2018)

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1: 1000
• Cell Tissue Type: human keratinocytes