Anticorps Polyclonal de lapin anti-EPHX2

• Phare
• Validé par KD/KO

EPHX2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10833-1-AP

Synonymes

CEH, Cytosolic epoxide hydrolase, EPHX2, Epoxide hydratase, Epoxide hydrolase 2, SEH, Soluble epoxide hydrolase

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Galerie de données de validation

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  WB analysis of A549 using 10833-1-AP

  WB result of EPHX2 antibody (10833-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.

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  WB analysis of HEK-293 using 10833-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 10833-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10833-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 10833-1-AP

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse large intestine using 10833-1-AP

  IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:300) with mouse large intestine tissue lysate 3200 ug.

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  IP experiment of HEK-293 using 10833-1-AP

  IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10833-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10833-1-AP (EPHX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HEK-293T, tissu de cervelet de rat, tissu de côlon de souris
• Résultats positifs en IP: tissu de gros intestin de souris, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

10833-1-AP cible EPHX2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPHX2 Protéine recombinante Ag1283
• Nom complet: epoxide hydrolase 2, cytoplasmic
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC013874
• Symbole du gène: EPHX2
• Identification du gène (NCBI): 2053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Publications

Species	Application	Title
mouse	WB,IF	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
human	WB	Int J Biol Sci; Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury; Authors - Juan Zhang
rat	WB	Biochim Biophys Acta Mol Basis Dis; Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ; Authors - Yang Zhou
human	WB,IHC	Front Oncol; EPHX2 Inhibits Colon Cancer Progression by Promoting Fatty Acid Degradation.; Authors - Yiran Zhou
chicken	WB	Arch Biochem Biophys; Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase.; Authors - Labitzke Erin M EM
rat	WB	Biochem Biophys Res Commun; Adeno-associated virus-mediated in vivo suppression of expression of EPHX2 gene modulates the activity of paraventricular nucleus neurons in spontaneously hypertensive rats.; Authors - Xiaoming Zhu