Anticorps Polyclonal de lapin anti-ENPP2

ENPP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 14243-1-AP

Synonymes

ATX, ATX X, Autotaxin, E NPP 2, ENPP2, LysoPLD, PD IALPHA, PDNP2

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Galerie de données de validation

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  WB analysis using 14243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14243-1-AP (Autotaxin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14243-1-AP (Autotaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14243-1-AP

  IP result of anti-Autotaxin (IP:14243-1-AP, 5ug; Detection:14243-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human small intestine using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50.

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  IHC staining of human liver cancer using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50.

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  IHC staining of human liver cancer using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu placentaire humain, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

14243-1-AP cible ENPP2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENPP2 Protéine recombinante Ag5499
• Nom complet: ectonucleotide pyrophosphatase/phosphodiesterase 2
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC034961
• Symbole du gène: ENPP2
• Identification du gène (NCBI): 5168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.; Authors - Jillian A Bristol
human,mouse	WB	Cells; Role of Aberrantly Activated Lysophosphatidic Acid Receptor 1 Signaling Mediated Inflammation in Renal Aging.; Authors - Yongjie Jin
mouse	WB	Front Pharmacol; UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of Cinnabar and Realgar.; Authors - Fangbo Xia
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
mouse	WB	Am J Physiol Cell Physiol; The Ecto-Nucleotide Pyrophosphatase/Phosphodiesterase 2 Promotes Early Osteoblast Differentiation and Mineralization in Stromal Stem Cells; Authors - Irina L Tourkova
mouse	WB	Mol Ther Oncolytics; Berberine inhibits carcinogenesis through antagonizing the ATX-LPA-LPAR2-p38-leptin axis in a mouse hepatoma model; Authors - Gang Ren

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jacqueline (Verified Customer) (05-14-2019)

Works perfectly in western blot using concentrated media of cells that are cultured in serum-depleted conditions. Serum is a good positive control or secreted ENPP2/ATX. In our hands ~120kD

• Applications: Western Blot, Immunoprecipitation, Cell culture,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Media from PDAC cells and fibroblasts

ENPP2 Antibody Western Blot,Immunoprecipitation,Cell culture, validation (1:500 dilution) in Media from PDAC cells and fibroblasts (Cat no:14243-1-AP)