Anticorps Polyclonal de lapin anti-ENOX2

• Phare
• Validé par KD/KO

ENOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 10423-1-AP

Synonymes

Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen

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Galerie de données de validation

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  WB analysis of MCF-7 using 10423-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10423-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10423-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10423-1-AP

  IP result of anti-ENOX2 (IP:10423-1-AP, 3ug; Detection:10423-1-AP 1:700) with MCF-7 cells lysate 4000ug.

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  IHC staining of human ovary tumor using 10423-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10423-1-AP (ENOX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10423-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10423-1-AP (ENOX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 10423-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ENOX2 antibody (10423-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules PC-3, tissu ovarien de souris
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IP: See 3 publications below

Informations sur le produit

10423-1-AP cible ENOX2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENOX2 Protéine recombinante Ag0674
• Nom complet: ecto-NOX disulfide-thiol exchanger 2
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC019254
• Symbole du gène: ENOX2
• Identification du gène (NCBI): 10495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock

Publications

Species	Application	Title
human	WB	J Med Chem; Synthesis and characterization of 4,11-diaminoanthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through tNOX-modulated NAD+/NADH ratio and SIRT1.; Authors - Alexander S Tikhomirov
human	IP	Am J Cancer Res; Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells.; Authors - Chin-Fang Chang
human,mouse	IP	Am J Cancer Res; Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells.; Authors - Atikul Islam
human	WB	Exp Cell Res; Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.; Authors - Zeng Zih-Ming ZM ; KD Validated
human	WB	J Agric Food Chem; Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo.; Authors - Liu Nei-Chi NC
human	WB	Biochem Biophys Res Commun; RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.; Authors - Liu Shan-Chi SC ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wassim (Verified Customer) (04-22-2019)

This is a western blot analysis of Enox2 in membrane fractions as seen in normal epithelial cells versus abnormal cells. The molecular weight of fractions is indicated above each lane.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Epithelial cells

ENOX2 Antibody Western Blot, validation (1:2000 dilution) in Epithelial cells (Cat no:10423-1-AP)