Anticorps Polyclonal de lapin anti-EML4

EML4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 22709-1-AP

Synonymes

C2orf2, ELP120, EMAP 4, EMAPL4, EML4, Ropp 120, ROPP120

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Galerie de données de validation

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  WB analysis of A549 using 22709-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22709-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22709-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 22709-1-AP

  IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.

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  IHC staining of human breast cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 22709-1-AP

  Immunofluorescent analysis of A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 22709-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HeLa
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22709-1-AP cible EML4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EML4 Protéine recombinante Ag18463
• Nom complet: echinoderm microtubule associated protein like 4
• Masse moléculaire calculée: 981 aa, 109 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC140845
• Symbole du gène: EML4
• Identification du gène (NCBI): 27436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.