Anticorps Polyclonal de lapin anti-EMG1

• Phare
• Validé par KD/KO

EMG1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11965-1-AP

Synonymes

C2F, EMG1, Grcc2f, NEP1, Nucleolar protein EMG1 homolog, Protein C2f

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Galerie de données de validation

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  WB analysis of HEK-293 using 11965-1-AP

  WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11965-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 11965-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11965-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11965-1-AP

  WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HeLa cells.

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  IP experiment of HeLa using 11965-1-AP

  IP result of anti-EMG1 (IP:11965-1-AP, 4ug; Detection:11965-1-AP 1:400) with HeLa cells lysate 1080ug.

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  IF Staining of HeLa using 11965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EMG1 antibody (11965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules PC-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11965-1-AP cible EMG1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMG1 Protéine recombinante Ag2575
• Nom complet: EMG1 nucleolar protein homolog (S. cerevisiae)
• Masse moléculaire calculée: 244 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC055314
• Symbole du gène: EMG1
• Identification du gène (NCBI): 10436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EMG1, also named as NEP1, is a highly conserved protein initially identified as "Essential for Mitotic Growth" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.

Publications

Species	Application	Title
		Mol Cell Proteomics; Nucleolar enrichment of brain proteins with critical roles in human neurodevelopment.; Authors - Lukasz Slomnicki
mouse	IF	Biochim Biophys Acta; Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression.; Authors - Joy Armistead
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun