Anticorps Polyclonal de lapin anti-ELF2

• Phare
• Validé par KD/KO

ELF2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12499-1-AP

Synonymes

E74 like factor 2, ELF2, ELF2A, EU32, NERF, NERF 1A, NERF 1a,b, NERF 1B, NERF 2, New ETS related factor

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Galerie de données de validation

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  WB analysis of A2780 using 12499-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12499-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12499-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12499-1-AP (ELF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 12499-1-AP

  IP result of anti-ELF2 (IP:12499-1-AP, 4ug; Detection:12499-1-AP 1:500) with A2780 cells lysate 960ug.

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Applications testées

• Résultats positifs en WB: cellules A2780, tissu cérébral humain, tissu hépatique humain
• Résultats positifs en IP: cellules A2780

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

12499-1-AP cible ELF2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELF2 Protéine recombinante Ag3170
• Nom complet: E74-like factor 2 (ets domain transcription factor)
• Masse moléculaire calculée: 533 aa, 57 kDa
• Poids moléculaire observé: 63-64 kDa, 56-57 kDa
• Numéro d’acquisition GenBank: BC034951
• Symbole du gène: ELF2
• Identification du gène (NCBI): 1998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.

Publications

Species	Application	Title
goat	WB	Front Cell Dev Biol; A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.; Authors - Yue Zhang ; KD Validated
human	WB	Cell Biol Int; Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2.; Authors - Yunpeng Bai
mouse	WB	Ren Fail; ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.; Authors - Shizhu Yuan