Anticorps Polyclonal de lapin anti-ELAVL2

• Phare
• Validé par KD/KO

ELAVL2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IP, RIP, ELISA, IF

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 14008-1-AP

Synonymes

HUB, Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 14008-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14008-1-AP (ELAVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14008-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14008-1-AP (ELAVL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 14008-1-AP

  IP result of anti-ELAVL2 (IP:14008-1-AP, 4ug; Detection:14008-1-AP 1:30000) with mouse cerebellum tissue lysate 3000 ug.

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  IHC staining of human brain using 14008-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14008-1-AP (ELAVL2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14008-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14008-1-AP (ELAVL2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris
• Résultats positifs en IP: tissu de cervelet de souris,
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• RIP: See 3 publications below

Informations sur le produit

14008-1-AP cible ELAVL2 dans les applications de WB, IHC, IP, RIP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELAVL2 Protéine recombinante Ag5105
• Nom complet: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC030692
• Symbole du gène: ELAVL2
• Identification du gène (NCBI): 1993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Publications

Species	Application	Title
mouse	IP	Sci Adv; Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development; Authors - Yuki Takada
human	WB,IF,RIP	Nucleic Acids Res; HuB and HuD repress telomerase activity by dissociating HuR from TERC.; Authors - Xiaolei Cheng ; KD Validated
mouse	RIP	EMBO Rep; ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles.; Authors - Yuzuru Kato
chicken	IF	Cell Rep; miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability.; Authors - Ronald J Parchem
human	WB	Hum Mol Genet; ELAVL2-regulated transcriptional and splicing networks in human neurons link neurodevelopment and autism.; Authors - Stefano Berto ; KD Validated
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jacopo (Verified Customer) (09-03-2019)

Very noce and specific bands in western blot. Also works for RIP

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma stem cells