Anticorps Polyclonal de lapin anti-ELA2

ELA2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 27642-1-AP

Synonymes

Bone marrow serine protease, ELA2, ELANE, Elastase 2, elastase 2, neutrophil, GE, HLE, HNE, Human leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase

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Galerie de données de validation

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  WB analysis using 27642-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27642-1-AP (ELA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27642-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 27642-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ELA2 antibody (27642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules U-937
• Résultats positifs en IHC: tissu de lymphome humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

27642-1-AP cible ELA2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELA2 Protéine recombinante Ag26634
• Nom complet: elastase 2, neutrophil
• Masse moléculaire calculée: 267 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC074816
• Symbole du gène: ELA2
• Identification du gène (NCBI): 1991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Mol Biol Lett; Obacunone alleviates ferroptosis during lipopolysaccharide-induced acute lung injury by upregulating Nrf2-dependent antioxidant responses.; Authors - Jin Li
bovine	IF	Animals (Basel); Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows.; Authors - Guanxin Lv
mouse	WB	Cancer Immunol Res; Tumor cell-derived microparticles induced by methotrexate augment T-cell antitumor responses by down-regulating expression of PD-1 in neutrophils; Authors - Pingwei Xu
rat	IHC	Front Pharmacol; RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis; Authors - Jiaqi Yao