Anticorps Polyclonal de lapin anti-EIF5B

• Phare
• Validé par KD/KO

EIF5B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 13527-1-AP

Synonymes

eIF 5B, EIF5B, IF2, KIAA0741

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Galerie de données de validation

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  WB analysis of mouse brain using 13527-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13527-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13527-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13527-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 13527-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below

Informations sur le produit

13527-1-AP cible EIF5B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF5B Protéine recombinante Ag4404
• Nom complet: eukaryotic translation initiation factor 5B
• Masse moléculaire calculée: 1220 aa, 139 kDa
• Poids moléculaire observé: 175 kDa
• Numéro d’acquisition GenBank: BC032639
• Symbole du gène: EIF5B
• Identification du gène (NCBI): 9669
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.; Authors - Eunah Kim
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang ; KD Validated
human	WB	bioRxiv; Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation; Authors - Yuanhui Mao ; KD Validated
human	WB	bioRxiv; LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins; Authors - James A Saba ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bill (Verified Customer) (12-23-2019)

This antibody give every strong signal on EIF5B.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colerectal cancer cells