Anticorps Polyclonal de lapin anti-EIF5A1/EIF5A2

• Phare
• Validé par KD/KO

EIF5A1/EIF5A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, RIP, ELISA, IF

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 17069-1-AP

Synonymes

eIF 5A 2, EIF 5A2, EIF5A1/EIF5A2, EIF5A2, eIF5AII

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Galerie de données de validation

• 

  WB analysis of mouse brain using 17069-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 17069-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17069-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17069-1-AP

  IP result of anti-EIF5A1/EIF5A2 (IP:17069-1-AP, 3ug; Detection:17069-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 17069-1-AP (EIF5A2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 17069-1-AP (EIF5A2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A2780, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

17069-1-AP cible EIF5A1/EIF5A2 dans les applications de WB, IHC, IP, RIP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF5A1/EIF5A2 Protéine recombinante Ag10895
• Nom complet: eukaryotic translation initiation factor 5A2
• Masse moléculaire calculée: 153 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC036072
• Symbole du gène: EIF5A2
• Identification du gène (NCBI): 56648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 5A (EIF5A) plays an essential role in the viability of eukaryotic cells. EIF5A is known to act as a translation initiation factor specific for a small number of mRNAs, a cellular target of HIV-1 REV protein, and an exportin-4-dependent nuclear export cargo. It is also involved in mRNA turnover and the establishment of actin polarity. [PMID:16157662]. EIF5A2, one isoform of EIF5A, has a key at the level of mRNA turnoverby acting downstream of decapping. It also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity [PMID:14622290]. EIF5A2 shares 84% identity of amino acid sequence with EIF5A1 isoform, so EIF5A2 poly-antibody could recognize both EIF5A2 and EIF5A1.

Publications

Species	Application	Title
mouse	WB,RIP	Oncogene; Identification of genes that promote PI3K pathway activation and prostate tumour formation; Authors - Jeffrey C Francis
mouse	WB	PLoS Biol; Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.; Authors - Dong Ma
mouse	WB	FASEB J; Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain.; Authors - Rebecca Chase
human	WB	J Mol Biol; Eukaryotic Initiation Factor 5A2 Regulates Expression of Antiviral Genes.; Authors - Dorian Farache
human	WB	PLoS One; Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.; Authors - Jin Yan Y
human	WB	Breast Cancer; EIF5A2 is a novel chemoresistance gene in breast cancer.; Authors - Yu Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah-Eve (Verified Customer) (02-19-2023)

Very Nice and specific signal, works well

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for wb and 1:200 for IF
• Cell Tissue Type: Human Pulmonary artery smooth muscle cells