Anticorps Polyclonal de lapin anti-EIF4G1

• Phare
• Validé par KD/KO

EIF4G1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 15704-1-AP

Synonymes

eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

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Galerie de données de validation

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  WB analysis of HeLa using 15704-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15704-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15704-1-AP

  IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15704-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15704-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15704-1-AP

  Immunofluorescent analysis of Hela cells, using EIF4G1 antibody 15704-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2, cellules HepG2 traitées à l'acide éthacrynique

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

15704-1-AP cible EIF4G1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF4G1 Protéine recombinante Ag8342
• Nom complet: eukaryotic translation initiation factor 4 gamma, 1
• Masse moléculaire calculée: 1600 aa, 176 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC007788
• Symbole du gène: EIF4G1
• Identification du gène (NCBI): 1981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Publications

Species	Application	Title
	CoIP	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	IF	J Extracell Vesicles; Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.; Authors - Bo You
human	WB	Proc Natl Acad Sci U S A; Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.; Authors - Brian C Avanzino
human	WB	PLoS Genet; SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.; Authors - Siqi Hu
mouse	WB,IF	Diabetes; Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.; Authors - Seokwon Jo ; KO Validated
human	IF	J Cell Sci; ADAR1 limits stress granule formation through both translation-dependent and translation-independent mechanisms.; Authors - Giulia A Corbet