Anticorps Polyclonal de lapin anti-EIF4A3

EIF4A3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10463-1-AP

Synonymes

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

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Galerie de données de validation

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  WB analysis of HeLa using 10463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10463-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 10463-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10463-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10463-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10463-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10463-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10463-1-AP

  IP result of anti-EIF4A3 (IP:10463-1-AP, 3ug; Detection:10463-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Raji, tissu hépatique de souris, tissu pulmonaire humain
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10463-1-AP cible EIF4A3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF4A3 Protéine recombinante Ag0733
• Nom complet: eukaryotic translation initiation factor 4A, isoform 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC004386
• Symbole du gène: EIF4A3
• Identification du gène (NCBI): 9775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Publications

Species	Application	Title
human	WB,IP	Genes Dev; SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.; Authors - Yamashita Akio A
human	WB	Sci Signal; AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.; Authors - Izumi Natsuko N