Anticorps Polyclonal de lapin anti-EIF4A2

EIF4A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11280-1-AP

Synonymes

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

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Galerie de données de validation

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  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11280-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 11280-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11280-1-AP

  IP result of anti-EIF4A2 (IP:11280-1-AP, 4ug; Detection:11280-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11280-1-AP (EIF4A2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, tissu ovarien de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11280-1-AP cible EIF4A2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF4A2 Protéine recombinante Ag1810
• Nom complet: eukaryotic translation initiation factor 4A, isoform 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015842
• Symbole du gène: EIF4A2
• Identification du gène (NCBI): 1974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Publications

Species	Application	Title
human	IHC, WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
mouse	WB	Stem Cell Rev Rep; Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation; Authors - Jian Ma
human	WB	Mol Carcinog; miR-181a/b Induces the Growth, Invasion and Metastasis of Neuroblastoma Cells Through Targeting ABI1.; Authors - Xiaodan Liu
mouse	WB,IF	Biol Reprod; Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.; Authors - Jianjun Hu
human	WB	Microbes Infect; eIF4A2 is a host factor required for efficient HIV-1 replication.; Authors - Jerry Kwame Ndzinu