Anticorps Polyclonal de lapin anti-EIF3D

• Phare
• Validé par KD/KO

EIF3D Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, levure, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA

Conjugaison

Non conjugué

Publications

20

Avis (3)

N° de cat : 10219-1-AP

Synonymes

Eukaryotic translation initiation factor 3 subunit 7, eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66

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Galerie de données de validation

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  WB analysis of mouse brain using 10219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 10219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10219-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10219-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10219-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10219-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10219-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10219-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10219-1-AP

  IP result of anti-EIF3D (IP:10219-1-AP, 4ug; Detection:10219-1-AP 1:300) with A549 cells lysate 2800 ug.

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  IHC staining of human breast cancer using 10219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10219-1-AP (EIF3D Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10219-1-AP (EIF3D Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining using 10219-1-AP

  IF result of 10219-1-AP (anti-EIF3D) in U2OS cell (treated with 100 mM sodium arsenite to cause stress-induced translational arrest) by Dr. Nancy Kedersha.U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with PTG anti-eIF3p66 in green; and counterstained with anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.

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  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3D antibody (10219-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 10219-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EIF3D antibody 10219-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of transfected cells using 10219-1-AP

  IF result of 10219-1-AP (anti-EIF3D) in U2OS cell by Dr. Nancy Kedersha. U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with 10219-1-AP (EIF3D) in green; and counterstained with Santa Cruz anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.; The EIF3D(p66) appears associated with mitochondria.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules HepG2, cellules Jurkat, cellules L02, tissu cérébral humain
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS, cellules HepG2, cellules HepG2 traitées à l'acide éthacrynique, cellules MCF-7, cellules transfectées

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

10219-1-AP cible EIF3D dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA et montre une réactivité avec des échantillons Humain, levure, souris

• Réactivité: Humain, levure, souris
• Réactivité citée: rat, Humain, Lapin, levure, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF3D Protéine recombinante Ag0268
• Nom complet: eukaryotic translation initiation factor 3, subunit D
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC000328
• Symbole du gène: EIF3D
• Identification du gène (NCBI): 8664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Publications

Species	Application	Title
human	WB	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
mouse	WB	Mol Cell; Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.; Authors - Zhen Shi
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
human	IHC	EBioMedicine; PMID: 31669222; Authors - Hai Huang
mouse	WB	Dev Cell; Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c; Authors - Kotaro Fujii
rabbit	WB	EMBO J; HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.; Authors - Locker Nicolas N

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tobias (Verified Customer) (10-25-2022)

clean and good antibody for wb

• Applications: Western Blot

FH

Peter (Verified Customer) (10-24-2022)

Specific band at correct MW

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549

FH

Joleen (Verified Customer) (06-10-2019)

EIF3D antibody recognizes a prominent band around the correct molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

EIF3D Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10219-1-AP)